Inflammatory Effect of Cleaved Bovine Lactoferrin by Elastase on Staphylococcal Mastitis

Journal of Veterinary Medical Science

Yumiko KOMINE⁵  Shunji SUGAWARA⁴  Yoshiaki OBARA³  Katsuo KUMAGAI⁶  Jin KOBAYASHI¹  Toshinobu KUROISHI⁵  Hisashi ASO²  Ken-ichi KOMINE⁵ 

[1] Department of Animal Reproduction, Miyagi University Research Farm;Cellular Biology Laboratory, Faculty of Agriculture, Tohoku University;Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University;Division of Oral Immunology, Department of Oral Biology, Tohoku University Graduate School of Dentistry;Intelligent Cosmos Research Institute Corporation;Department of Microbiology, Tohoku University Graduate School of Dentistry

关键词: concanavalin A;    elastase;    lactoferrin;    mastitis;    Staphylococcus aureus;   

DOI  :  10.1292/jvms.68.715

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Cited-By(1)Elastase activity and concanavalin A (Con A) low affinity bovine lactoferrin (bLf) molecule were detected in mammary gland secretions (MGSs) from mammary glands (MGs) with clinical staphylococcal mastitis. Changes in clinical symptoms correlated with increases in both elastase activity and the concentration of Con A low-affinity Lf in MGSs from mastitic MGs. Bovine Lf treated with elastase (elastase-Lf) showed various small bLf molecules and the same image on Con A two-dimensional immunoelectrophoresis as low Con A affinity bLf in MGSs. We confirmed the presence of four common bLf peptides for the elastase-bLf and low Con A affinity bLf molecules in mastitic MGSs, and synthesized four peptides. Strong mRNA expression of interleukin-6 (IL-6), tumor necrosis factor α (TNFα), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) was induced in bovine mammary epithelial cells on stimulation with low Con A affinity bLf, elastase-bLf, and GQRDLLFKDSAL, a synthesis bLf peptide based on nuclear factor kappa B (NFκB) activation. These results suggest that bLf was cleaved by elastase, and that this cleavage changed the physical function of Lf. Our results indicate that elastase induced production of low Con A affinity bLf, including the bLf peptide GQRDLLFKDSAL, and had an inflammatory effect on staphylococcal mastitis.

【 授权许可】

Unknown