Experimental infection of coho salmon Oncorhynchus kisutch by exposure of skin, gills and intestine with Piscirickettsia salmonis

Diseases of Aquatic Organisms

P. A. Smith¹  A. Guajardo¹  J. Contreras¹  M. A. Morales¹  M. E. Rojas¹  J. Larenas¹ 

关键词: Piscirickettsia salmonis;    Pathogenesis;    Fish disease;    Coho salmon;   

DOI  :  10.3354/dao061053

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Piscirickettsiosis pathogenesis was examined using some tissues as entry portals of Piscirickettsia salmonis in coho salmon. Juvenile fish, weighing approximately 8.4 g, were used in this trial. Inocula were prepared using the strainSLGO-95 of P. salmonis. The micro-organism was cultured in the CHSE-214 cell line as described by Fryer et al. (1990) and doses containing 10^4.7 and 10^3.7 TCID₅₀ were prepared. Each dose was used to infect the fishvia skin, gills and intestine. Skin and gills were exposed by calibrated drops, and the intestine by an intubation through the anal opening. Some fish were injected intraperitoneally with the same P. salmonis doses, as positive virulence controls.Sham-inoculated fish for each of the tested routes were also included as negative controls. Piscirickettsiosis was experimentally reproduced with all the inoculation methods. Cumulative mortalities and survival analyses showed that the most effectiveentry portal was skin followed by intestinal intubation and finally by gill infection.

【 授权许可】

Unknown