Identification and Characterization of CYP2B6 cDNA in Cynomolgus Macaques (Macaca fascicularis)

Journal of Veterinary Medical Science

Masahiro UTOH¹  Kiyomi MATSUNO¹  Yasuhiro UNO¹  Chika NAKAMURA¹  Hiroshi YAMAZAKI² 

[1] Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL);Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University

关键词: cloning;    liver;    cytochrome P450;    monkey;   

DOI  :  10.1292/jvms.001653

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(6)Cytochrome P450 2B6 (CYP2B6), an important drug-metabolizing enzyme, is involved in the metabolism of prescribed drugs in humans. Despite its importance, cDNA for a CYP2B6 ortholog has not been identified and characterized in cynomolgus macaques, which are frequently used in preclinical studies. In this study, cDNA highly homologous to human CYP2B6 was cloned from the cynomolgus macaque liver. This cDNA contained an open reading frame of 491 amino acids and functional domains characteristic for CYP protein, such as substrate recognition sites and a heme-binding region. Cynomolgus CYP2B6 was expressed predominantly in the liver with some extra-hepatic expression among 10 tissues. Moreover, cynomolgus CYP2B6 revealed activities toward testosterone 16β-hydroxylation and bupropion hydroxylation. These results suggest that cynomolgus CYP2B6 has a functional role in the liver.

【 授权许可】

Unknown