Identification and Characterization of CYP2C18 in the Cynomolgus Macaque (Macaca fascicularis)

Journal of Veterinary Medical Science

Masahiro UTOH¹  Kiyomi MATSUNO¹  Yasuhiro UNO¹  Chika NAKAMURA¹  Hiroshi YAMAZAKI² 

[1] Pharmacokinetics and Bioanalysis Center (PBC), Shin Nippon Biomedical Laboratories (SNBL);Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University

关键词: cloning;    CYP2C18;    cytochrome P450;    liver;    monkey;   

DOI  :  10.1292/jvms.09-0341

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(4)The macaque is widely used for investigation of drug metabolism due to its evolutionary closeness to the human. However, the genetic backgrounds of drug-metabolizing enzymes have not been fully investigated; therefore, identification and characterization of drug-metabolizing enzyme genes are important for understanding drug metabolism in this species. In this study, we isolated and characterized a novel cytochrome P450 2C18 (CYP2C18) cDNA in cynomolgus macaques. This cDNA was highly homologous (96%) to human CYP2C18 cDNA. Cynomolgus CYP2C18 was preferentially expressed in the liver and kidney. Moreover, a metabolic assay using cynomolgus CYP2C18 protein heterologously expressed in Escherichia coli revealed its activity toward S-mephenytoin 4'-hydroxylation. These results suggest that cynomolgus CYP2C18 could function as a drug-metabolizing enzyme in the liver.

【 授权许可】

Unknown