Development of a genotyping tool for a functionally relevantCYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgusmacaques

Journal of Veterinary Medical Science

Yasuhiro UNO¹  Hiroshi YAMAZAKI² 

[1] Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642�?0017, Japan;Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194�?8543, Japan

关键词: CYP2C19;    cytochrome P450;    genotyping;    monkey;    polymorphisms;   

DOI  :  10.1292/jvms.15-0416

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(3)Supplementary materials(1).In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19(formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2Csubstrates and is thus an important drug-metabolizing enzyme. One of the cynomolgusCYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p.Ile112Leu) results insubstantially reduced metabolic activity and thus is an important allele in drugmetabolism studies. For this allele, a genotyping tool was developed using allele-specificTaqMan probe. Genotyping 40 Cambodian cynomolgus macaques using this tool found 1homozygote, 17 heterozygotes and 22 wild type animals, and the result was confirmed bydirect-sequencing. Interestingly, this allele frequency was similar to that of Chinesecynomolgus macaques. The genotyping tool established is useful for drug metabolism studiesusing cynomolgus macaques.

【 授权许可】

Unknown