Journal of Veterinary Medical Science | |
Development of a genotyping tool for a functionally relevantCYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgusmacaques | |
Yasuhiro UNO1  Hiroshi YAMAZAKI2  | |
[1] Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642�?0017, Japan;Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo 194�?8543, Japan | |
关键词: CYP2C19; cytochrome P450; genotyping; monkey; polymorphisms; | |
DOI : 10.1292/jvms.15-0416 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(11)Cited-By(3)Supplementary materials(1).In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19(formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2Csubstrates and is thus an important drug-metabolizing enzyme. One of the cynomolgusCYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p.Ile112Leu) results insubstantially reduced metabolic activity and thus is an important allele in drugmetabolism studies. For this allele, a genotyping tool was developed using allele-specificTaqMan probe. Genotyping 40 Cambodian cynomolgus macaques using this tool found 1homozygote, 17 heterozygotes and 22 wild type animals, and the result was confirmed bydirect-sequencing. Interestingly, this allele frequency was similar to that of Chinesecynomolgus macaques. The genotyping tool established is useful for drug metabolism studiesusing cynomolgus macaques.
【 授权许可】
Unknown
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