Influences of Organic Solvents on CYPMPO-Electron Spin Resonance Spectra in In Vitro Radical Generating Systems

Journal of Veterinary Medical Science

Masato KAMIBAYASHI³  Masashi MUKOHDA²  Hideyuki YAMAWAKI²  Yukio HARA²  Shunji UENO¹  Muneyoshi OKADA² 

[1] Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University;Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University;Kyoto Pharmaceutical University

关键词: acetonitrile;    CYPMPO;    electron spin resonance;    organic solvents;   

DOI  :  10.1292/jvms.10-0232

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(7)Estimation of radical scavenging capacity of lipophilic antioxidants by electron spin resonance (ESR) in vitro is a challenging issue due to their poor solubility in aqueous radical generating and measuring systems. Water-miscible organic solvents are used for this purpose. A novel radical trapping agent, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO), that has practical advantages over well-known trapping agents was synthesized. However, no available data for the influence of solvents in an ESR system that uses CYPMPO has been presented. The influences of six water-miscible organic solvents, acetonitrile (AcN), acetone, dimethyl sulfoxide (DMSO), ethanol, polyethylene glycol (PEG), and dimethoxyethane (DME), on ESR responses to Fenton Fe2+/H 2O2 OH· and hypoxanthine/xanthine oxidase superoxide generation systems in vitro were studied. Reduction of the ESR signal to CYPMPO-OH· adducts by 55.86 ± 5.95 and 83.17 ± 2.50% compared with the control was observed in the presence of AcN and acetone, respectively, at a final concentration of 5% (v/v). AcN of less than 1% had minimal effects. DMSO, ethanol, PEG and DME at 5% (v/v) strongly inhibited the ESR signals and/or caused derangement in the signal patterns. The six water-miscible solvents at 5% (v/v) had no influence on the ESR spectra of CYPMPO-superoxide adducts. From these results, AcN, at less than 1% (v/v), is a useful water-miscible organic solvent for assessing radical scavenging capacities of lipophilic compounds in the CYPMPO-Fenton Fe2+/H2O2 OH· reaction system in an ESR assay. Any of the solvents used in the present study can be used in a hypoxanthine/xanthine oxidase superoxide generation system.

【 授权许可】

Unknown