Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Brazilian Journal of Infectious Diseases

Alves, Ronaldo Mendes¹  Campos, Leila Carvalho¹  Seki, Liliane Miyuki¹  Vallim, Deyse Christina¹  Chagas, Thiago Pavoni Gomes²  Asensi, Marise Dutra² 

[1] Fiocruz, Rio de Janeiro, Brazil

关键词: Klebsiella pneumoniae;    β;    -lactamases;    genotype.;   

DOI  :  10.1590/S1413-86702011000500002

来源: Contexto

【 摘 要 】

OBJECTIVE: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil.
MATERIAL AND METHODS: Eighty-five K.pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC₅₀ and MIC₉₀ for ESBL-producing isolates were determined by the Etest^® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for bla_TEM, bla_SHV, bla_CTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE.
RESULTS AND DISCUSSION: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K.pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.

【 授权许可】

CC BY-NC-ND