Nucleotide sequence analysis of the glycoprotein gene of putative spring viraemia of carp virus and pike fry rhabdovirus isolates reveals four genogroups

Diseases of Aquatic Organisms

K. Way¹  A. M. Sheppard¹  G. R. Taylor¹  K. L. Denham¹  C. T.-Y. Liu¹  D. M. Stone¹  W. Ahne¹  P. F. Dixon¹ 

关键词: Rhabdovirus;    PFRV;    SVCV;    Sequence analysis;    Genogroups;   

DOI  :  10.3354/dao053203

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: RT-PCR methods have been applied to the detection and sequencing of the glycoprotein gene of putative spring viraemia of carp viruses (SVCV) and pike fry rhabdoviruses (PFRV), including isolates from tench, grass carp, roach, bream and falseharlequin, sheatfish and orfe. Phylogenetic analysis of a 550 nucleotide (nt) region of the glycoprotein gene identified 4 groups, I to IV. Significantly, the majority of viruses previously identified as PFRV formed a distinct cluster (Genogroup IV) whichshared <80% nucleotide identity with the PFRV reference strain (Genogroup III). The similarity between another PFRV-like virus isolated from grass carp and representatives of Genogroups III and IV was also <80%, indicating that this virus belongedto a third group (Genogroup II). All of the putative SVC viruses were assigned to a 4th group (Genogroup I), sharing <61% nucleotide identity with viruses in Genogroups II to IV.

【 授权许可】

Unknown