Genotyping spring viraemia of carp virus and other piscine vesiculo-like viruses using reverse hybridisation

Diseases of Aquatic Organisms

K. Way¹  A. M. Sheppard¹  D. M. Stone¹  P. D. Martin¹  G. Woolford¹  R.-M. Le Deuff¹ 

关键词: Rhabdovirus;    Spring viraemia of carp virus;    SVCV;    Vesiculovirus;    Genotyping;    Reverse hybridisation;   

DOI  :  10.3354/dao076163

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A simple nylon membrane-based DNA macroarray was developed to genotype spring viraemia of carp virus (SVCV) and related viruses. Twenty-six viruses were genotyped using the array, and the results were confirmed by phylogenetic analysis of a 426 bp partial glycoprotein gene sequence. The array was not only capable of discriminating between the 4 main genogroups of cyprinid vesiculo-type viruses described previously, but also accurately sub-type the SVC viruses assigned to Genogroup I. The assay offers a practical solution for diagnostic laboratories that currently lack a sequencing capability to confirm the nature of PCR products generated in suspected SVCV cases.

【 授权许可】

Unknown