Akabane Virus Utilizes Alternative Endocytic Pathways to Entry into MammalianCell Lines

Journal of Veterinary Medical Science

Akiko TAKENAKA-UEMA¹  Norasuthi BANGPHOOMI¹  Taisuke HORIMOTO¹  Hiroomi AKASHI¹  Kentaro KATO¹  Tatsuki SUGI¹ 

[1] Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan

关键词: Akabane virus;    caveolae;    clathrin;    endocytosis;   

DOI  :  10.1292/jvms.14-0155

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(41)Cited-By(2)The entry mechanisms ofAkabane virus (AKAV), Bunyaviridae family, have not yet been determined.In this study, chemical inhibitors were used to analyze endocytic mechanisms during AKAVinfection of mammalian cell lines. The analyses using drug treatments followed byquantitative measurement of viral RNA and N protein revealed that AKAV entersnon-bovine-derived cell lines (Vero, HmLu-1 and BHK cells) in a manner indicative ofclathrin endocytosis. By contrast, AKAV infection in bovine-derived cell lines (LB9.K andMDBK cells) is independent of this pathway. Further analyses indicated that AKAV entryinto bovine cell lines involves a non-clathrin, non-caveolae endocytic pathway that isdependent on dynamin. We conclude that although both cell types require a low pH for AKAVpenetration, AKAV utilizes alternative entry pathways into mammalian cell lines.

【 授权许可】

Unknown