Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at theMaternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of GoldNanoparticles

Journal of Veterinary Medical Science

Tatsuya HASEGAWA¹  Masashi SAKURAI⁶  Atsushi ASANO⁵  Akinori SHIMADA⁴  Takehito MORITA⁶  Hirohisa TAKANO³  Kasem RATTANAPINYOPITUK⁶  Kenichiro INOUE² 

[1] Department of Environmental Biochemistry, Yamanashi Institute of Environmental Sciences, 5597-1 Fujiyoshida, Yamanashi 403�?0005, Japan;Basic Medical Research Center, International University of Health and Welfare, 2600�?1 Kitakanamura, Ootawara-shi, Tochigi 324�?8501, Japan;Department of Environmental Engineering, Kyoto University Graduate School of Engineering, Kyoto Daigaku Katsura, Saikyo-ku, Kyoto 615�?8530, Japan;Laboratory of Pathology, School of Life and Environmental Science, Azabu University, 1�?17�?71 Fuchinobe, Sagamihara-shi, Kanagawa 252�?5201, Japan;Department of Veterinary Biochemistry, Tottori University, 4�?101 Koyama Minami, Tottori 680�?8553, Japan;Department of Veterinary Pathology, Tottori University, 4�?101 Koyama Minami, Tottori 680�?8553, Japan

关键词: caveolin;    clathrin;    endocytosis;    gold nanoparticles;    placenta;   

DOI  :  10.1292/jvms.13-0512

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(40)Cited-By(13)Exposure to nanoparticles during pregnancy is a public concern, becausenanoparticles may pass from the mother to the fetus across the placenta. The purpose ofthis study was to determine the possible translocation pathway of gold nanoparticlesacross the maternal–fetal barrier as well as the toxicity of intravenously administeredgold nanoparticles to the placenta and fetus. Pregnant ICR mice were intravenouslyinjected with 0.01% of 20- and 50-nm gold nanoparticle solutions on the 16th and 17th daysof gestation. There was no sign of toxic damage to the placentas as well as maternal andfetal organs of the mice treated with 20- and 50-nm gold nanoparticles. ICP-MS analysisdemonstrated significant amounts of gold deposited in the maternal livers and placentas,but no detectable level of gold in the fetal organs. However, electron microscopydemonstrated an increase of endocytic vesicles in the cytoplasm of syncytiotrophoblastsand fetal endothelial cells in the maternal–fetal barrier of mice treated with goldnanoparticles. Clathrin immunohistochemistry and immunoblotting showed increasedimmunoreactivity of clathrin protein in the placental tissues of mice treated with 20- and50-nm gold nanoparticles; clathrin immunopositivity was observed in syncytiotrophoblastsand fetal endothelial cells. In contrast, caveolin-1 immunopositivity was observedexclusively in the fetal endothelium. These findings suggested that intravenousadministration of gold nanoparticles may upregulate clathrin- and caveolin-mediatedendocytosis at the maternal–fetal barrier in mouse placenta.

【 授权许可】

Unknown