Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression

Diseases of Aquatic Organisms

Paisarn Sithigorngul¹  Sombat Rukpratanporn¹  Weerawan Sithigorngul¹  Parin Chaivisuthangkura¹  Siwaporn Longyant¹  Thanawan Tejangkura¹ 

关键词: Immunohistochemistry;    Litopenaeus vannamei;    Polyclonal antibody;    VP1;    VP3;    Western blot;    Taura syndrome virus;    TSV;   

DOI  :  10.3354/dao069249

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Capsid protein genes VP1 and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification.

【 授权许可】

Unknown