Essential oil of Tagetes filifolia against the four beetle Tribolium castaneum and its relation to acetylcholinesterase activity and lipid peroxidation

Agriscientia

Herrera, J.M²  Zygadlo, J.A²  Dambolena, J.S²  Zunino, M.P²  Lucini, E.I¹  Olmedo, R¹  Pizzolitto, R.P² 

[1] Universidad Nacional de Córdoba, Córdoba, Argentina;Universidad Nacional de Córdoba

关键词: Fumigant toxicity;    Tagetes filifolia;    Tribolium castaneum;    Lipid peroxidation;    Acetylcholinesterase activity;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Cordoba * Facultad de Ciencias Agropecuarias

【 摘 要 】

El desarrollo de insecticidas naturales podría ayudar a disminuir el impacto negativo de insecticidas sintéticos. La acción insecticida fumigante del aceite esencial (AEs) de Tagetes filifolia Lag (Asterales: Asteraceae) y sus compuestos principales fueron evaluados contra adultos de Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Los aceites esenciales fueron analizados por cromatografía gaseosa-espectrometría de masas. El aceite esencial y (E)-anetol mostraron mayor toxicidad contra insectos adultos a las 24 h (CL50 = 2,4 y 2,6 µL/mL water, respectivamente). Se evidenciaron cambios en los productos de peroxidación lipídica por un incremento en el contenido de malondialdehído. También se estudió la actividad in vitro de la enzima acetilcolinesterasa de insectos adultos de T. castaneum. (E)-anetol y estragol mostraron similar inhibición de la enzima (54 y 63%, respectivamente a 5 mM). El presente estudio constituye una de las primeras contribuciones para comprender la relación entre la acción insecticida del aceite esencial y el estrés oxidativo.Palabras clave: Toxicidad fumigante; Tagetes filifolia; Tribolium castaneum; Peroxidación de lípidos; Actividad acetilcolinesterasa.Fecha de recepción: 21/04/15;fecha de aceptación: 24/11/15  INTRODUCTIONThe flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) is a serious insect pest of stored products around the world. Synthetic insecticides such as methyl bromide and phosphine are used for stored-product protection. Current control measures for this species have several limitations. Methyl bromide affects the ozone layer thus, it has been banned in many countries and there is a commitment to reduce its use in 2015 (Arthur, 2012; Rajashekar et al., 2012). The inadequate and sustained use of phosphine enabled the emergence of resistance in insect populations (Jagadeesan et al., 2012; Opit et al. 2012). Recent studies indicated high levels of phosphine resistance in Brazilian populations of T. castaneum (Lorini et al., 2007; Pimentel et al., 2007). Although synthetic pesticides are effective, its frequent use has led to the presence of toxic residues in the environment and in food, and then generates negative effects on human health. These problems have highlighted the need to develop new and safe alternatives based on reducing the use of organophosphate, carbamates and chlorinated insecticides and promote the use of new techniques and products designed to reduce the resistance of insects and their presence in food. Thus, essential oils (EOs) obtained from aromatic plants are recognized as an alternative to synthetic insecticides against insect pest in stored foods (Rajashekar et al., 2012; Regnault-Roger et al., 2012). Plant EOs are promising in that they are easily biodegradable and have not described resistance. With a few exceptions, their mammalian toxicity is low and environmental persistence is short (Regnault- Roger et al., 2012). The genus Tagetes L. includes species with medicinal properties and their EOs have insecticidal activity (Krishna et al., 2005; Caballero-Gallardo et al., 2011; López et al., 2011; Stefanazzi et al., 2011). Among Tagetes species, particularly T. filifolia (Asteraceae, Helenieae) showed different degree in their insecticidal activity between different groups of insects (Camarillo et al., 2009; López et al. 2011).The insecticidal activity of EOs and their components can affect different and specific targets (Enan, 2001, 2005; Jbilou et al., 2008; Abdelgaleil et al., 2009; Rattan, 2010; Waliwitiya et al., 2010). However, the inhibitory effect of phenylpropenes on T. castaneum acetylcholinesterase (AChE) of adults, were not reported. Moreover, like synthetic pesticides, EOs could generate oxidative stress. The products of lipid degradation and decomposition are used in assessing oxidative stress, including conjugated dienes (CD), as well as the widely used product malondialdehyde (MDA (Zunino & Zygadlo, 2004).Therefore, in the present paper, we investigated the insecticidal activity of T. filifolia EO and their major components against T. castaneum. Changes in the products of lipid peroxidation, MDA and CD as indicators of oxidative stress are also discussed. The inhibitory effect on AChE activity of the adult insect was also evaluated. MATERIALS AND ME

【 授权许可】

CC BY-NC