Hyperactivation and Acrosome Reaction In Vitro in Spermatozoa Ejaculated by Cryptorchid Dogs after Orchiopexy

Journal of Veterinary Medical Science

Toshihiko TSUTSUI¹  Miyuki TAMURA¹  Akira OGASA¹  Eiichi KAWAKAMI¹  Michiru OGASAWARA¹  Harumichi NAITOH¹  Junichi HASEGAWA¹ 

关键词: capacitation;    cryptorchidism;    dog;    spermatozoa;   

DOI  :  10.1292/jvms.53.447

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(14)Orchiopexy of the cryptorchid (CR) testis and castration of the scrotal testis were performed in three unilaterally CR beagles at six months of age. Induction rates for ejaculated sperm hyperactivation (HA) and the acrosome reaction (AR) in vitro in these orchiopexied dogs were compared with five those in normal beagles one year later. Canine spermatozoa were incubated for 9 hr at 38°C under 5% CO2 in air in canine capacitation medium at a concentration of 30×106 sperm/ml. HA was observed using high-speed videomicrography. The AR spermatozoa were evaluated by the triple stain technique. As a result, there was no significant difference between 'the CR dogs after orchiopexy' (CDO) and the normal dogs (ND) with respect to sperm motility just after ejaculation. However, sperm motility of CDO decreased markedly during incubation. There was a significant difference in sperm motility between CDO (Mean±SD; 47±12%) and ND (80±9%) after three hours of incubation (p<0.01). No significant difference was observed between CDO and ND with respect to the HA rate of motile spermatozoa throughout the incubation period. The peak of HA rate was found in both CDO (58±5%) and ND (61±16%) after seven hours of incubation. The AR rate of spermatozoa in CDO was lower than that in ND after six hours of incubation. The AR rate of CDO (26±4%) was significantly lower than ND (46±5%) after eight hours of incubation (p<0.01). It is assumed that there might be relation between a rapid decrease of motility and low AR rate in spermatozoa of CDO during incubation.

【 授权许可】

Unknown