Molecular Cloning of cDNA for Equine Ovarian Inhibin/Activin βA Subunit

Journal of Veterinary Medical Science

Kyu-Tae CHANG¹  Keitaro YAMANOUCHI¹  Telhisa HASEGAWA¹  Michio TAKAHASHI¹  Masugi NISHIHARA¹  Shigemi MATSUYAMA¹  Akihiro IKEDA¹  Shintarou YOSHIDA¹  Masatoshi SUZUKI¹ 

关键词: activin;    cDNA cloning;    equine;    inhibin;    ovary;   

DOI  :  10.1292/jvms.57.469

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(6)cDNAs encoding equine inhibin/activin βA subunit were isolated from an equine follicle cDNA library and characterized. Using primers based on the rat inhibin/activin βA subunit cDNA sequence, a RT-PCR was performed to generate the probe for screening. Four positive clones were isolated. Analysis of the nucleotide sequence of these clones revealed that two pairs of identical clones were present, Eq-βA-1 (0.9 kb) and Eq-βA-2 (1.5 kb). Eq-βA-2 clone contained a complete open reading frame encoding 426amino acids. The deduced amino acid sequence of equine inhibin/activin βA subunit showed high similarity (>90%) to those of five other mammalian species. Northern blot analysis revealed that placenta from mare on day 180 of pregnancy contained a 1.5 kb inhibin/activin βA subunit mRNA.

【 授权许可】

Unknown