Histochemical Localization of Inhibin and Activin α, βA and βB Subunits in Rat Gonads

Journal of Veterinary Medical Science

Kenji OGAWA¹  Masamichi KUROHMARU¹  Takao NISHIDA¹  Kunio SHIOTA¹  Michio TAKAHASHI¹  Yoshihiro HAYASHI¹ 

关键词: activin;    inhibin;    ovary;    rat;    testis;   

DOI  :  10.1292/jvms.53.207

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(10)The localization of α, βA and βB subunits of inhibin/activin polypeptides was studied in the ovary and testis of sexually mature, immature, and embryonic rats. Specific staining with these three subunits was also evident in the oocytes from embryonic to mature female rats. This result suggests that inhibin- and activin-like substances may be produced in the oocytes and these substances may play a role in the oocyte growth and differentiation. Judging from the intensity of immunoreaction in mature female rats, the three subunits should be produced more abundantly in luteal cells than in the granulosa cells. Immunoreactive α, βA and βB subunits were observed in the cummulus oophorous in the morning (11:00), but not in the evening (23:00) on proestrus. The results are in well agreement with the previous report that inhibin α and βA subunit mRNA signals decline on proestrus evening. It is supposed that the cyclic change may be related with physiological phenomena prior to the ovulation, such as primary gonadotropin surges, loss of cummulus-oocyte gap junctions, or germinal vesicle breakdown. In both germ cells and Sertoli cells of the testis, α, βA and βB subunits were more abundant in the embryonic rat than in the mature rat. Although clear reactions with βA and βB subunits were detected in Leydig cells, α subunit was not detectable in the cells throughout the developmental stages examined.

【 授权许可】

Unknown