Molecular Cloning and Tissue Distribution of Uncoupling Protein 1 (UCP1) in Plateau Pika (Ochotona dauurica)

Journal of Veterinary Medical Science

Yuko OKAMATSU-OGURA¹  Kazuhiro KIMURA¹  Masayuki SAITO¹  Takahiro YAHATA³  Asako OMACHI¹  Takaaki MATSUMOTO²  Naoya KITAO¹ 

[1] Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Laboratory for Exercise Physiology and Biomechanics, School of Health and Sport Sciences, Chukyo University;Department of Nutritional Sciences, Faculty of Health and Welfare Science, Nayoro City University

关键词: brown adipose tissue;    plateau pika;    uncoupling protein;   

DOI  :  10.1292/jvms.69.1065

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Uncoupling protein 1 (UCP1) is present exclusively in brown adipose tissue, and contributes to body temperature control during cold exposure. We cloned UCP1 cDNA of plateau pika (Ochotona dauurica), a small, non-hibernating, diurnal lagomorph that inhabits in relatively cold climates and at high altitudes in Mongolia and in northern China. The nucleotide sequence of pika UCP1 was highly homologous to UCP1 of other species, and the deduced amino acid sequence had some common domains for UCP, including six mitochondrial carrier protein motifs and a putative purine-nucleotide binding site. RT-PCR and Western blot analyses revealed that both UCP1 mRNA and protein were expressed exclusively in the interscapular adipose tissue. These results suggest that pika UCP1 contributes to heat production in brown adipose tissue, as do those in other species.

【 授权许可】

Unknown