Regulation of Immunoreactive Activin A Secretion from Cultured Rat Anterior Pituitary Cells

Endocrine Journal

YUKIHIRO SAKAMOTO¹  YASUMI SHINTANI¹  KAZUYO HARADA¹  MAKI WAKATSUKI¹  ZHONG-HUI LIU¹  SHIRO SAITO¹  CHEN-YU ZHANG¹ 

[1] First Department of Internal Medicine, The University of Tokushima

关键词: Activin;    Radioimmunoassay;    Follicle-stimulating hormone;    Luteinizing hormone-releasing hormone;    Anterior pituitary cells;    Protein kinase C;   

DOI  :  10.1507/endocrj.43.39

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(27)Cited-By(9)Immunoreactive activin A (ir-activin A) release from cultured rat anterior pituitary cells was examined by measuring iractivin A in culture medium by a specific radioimmunoassay. Ir-activin A release into the medium increased over 1-18 days, and reached a maximal level at 12-15 days. The basal levels of ir-activin A in the culture media were 0.70±0.10 (mean±SD), 1.30±0.36 and 1.83±0.44ng/106 cells, when cultured for 6 days with 0, 2 and 10% fetal calf serum, respectively. LHRH induced an approximate 1.4-fold increase in ir-activin A release in contrast to a 40-60% inhibition with FSH, but LH did not affect the activin A release. In the presence of 12-o-tetradecanoylphorbol acetate (TPA), ir-activin A release was enhanced, but no significant effect was induced by forskolin. Activin A was distinctly immunostained in cultured rat anterior pituitary cells. These results suggested that activin A release from the pituitary is modified by FSH and LHRH, and that the activation of protein kinase C may be involved in the action of LHRH.

【 授权许可】

Unknown