Nuclear Overhauser effects in aqueous solution as dynamic probes in short linear peptides

FEBS Letters

Herranz, J.¹  Santoro, J.¹  Rico, M.¹  Nieto, J.L.¹  Jiménez, M.A.¹ 

[1] Instituto de Estructura de la Materia, CSIC, Serrano 119, 28006 Madrid, Spain

关键词: NOE;    Peptide flexibility;    NMR assignment;    Luteinizing hormone-releasing hormone;    RNase S-peptide;    NOE;    nuclear Overhauser effect;    NOESY;    two-dimensional nuclear Overhauser and exchange spectroscopy;    COSY;    two-dimensional homonuclear correlated spectroscopy;    LH-RH;    luteinizing hormone-releasing hormone;    RNase S-peptide;    1–19 fragment of bovine pancreatic ribonuclease A (EC 3.1.27.5);   

DOI  :  10.1016/0014-5793(88)80550-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility of obtaining interresidue NOEs from short linear peptides in aqueous solution has been investigated from an experimental point of view using peptides of various lengths (namely GGRA, LHRH and RNase S-peptide). It is shown that, provided that long (8̃00 ms) NOESY mixing times are used, complete sets of sequential αN NOEs are obtainable. From the intensities and signs of the observed NOEs, the relative mobilities of different parts of the polypeptide chain can be determined.

【 授权许可】

Unknown