A Direct Time-Resolved Fluoroimmunoassay (TR-FIA) for Measuring Plasma Estradiol-17β Concentrations in Cattle

Journal of Veterinary Medical Science

Kei IMAI¹  Kazuyoshi HASHIZUME²  Sanae HAMANAKA¹  Toru TAKAHASHI¹ 

[1] National Institute of Agrobiological Sciences;Iwate University

关键词: direct assay;    estradiol-17β;    TR-FIA;   

DOI  :  10.1292/jvms.66.225

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(9)A direct Time-Resolved Fluoroimmunoassay (TR-FIA) system for measuring estradiol-17β (E2) in bovine plasma was developed and evaluated. A 100 μl sample of bovine plasma was used for a TR-FIA without prior extraction and purification. The dose-response curves of reference standards ranged from 0.0625 to 10 pg/well. The minimum detectable concentration of this assay system was 0.625 pg/ml, and 19 pg/ml of E2 caused a 50% reduction of maximum binding. The intra- and inter-assay coefficients of variation were 10.2 and 17.4%, respectively. The plasma E2 concentrations measured by direct TR-FIA correlated closely with those measured after extraction (r=0.939). The results in the present study indicate that the TR-FIA reagent for E2, designed for human research can also be utilized, with some modification, for direct assaying in bovine plasma. This assay type seems to fulfill the requirements for safety, sensitivity, specificity, reproducibility and practical convenience.

【 授权许可】

Unknown