Measurement of Porcine Luteinizing Hormone Concentration in Blood by Time-Resolved Fluoroimmunoassay

Journal of Veterinary Medical Science

Chie SUZUKI³  Michiko NOGUCHI¹  Yasunori WADA¹  Sachiko ARAI¹  Seigo ITOH¹  Shokichi IWAMURA³  Toru TAKAHASHI²  Koji YOSHIOKA³  Hiroyuki KANEKO² 

[1] Laboratory of Veterinary Internal Medicine, Azabu University;National Institute of Agrobiological Sciences;National Institute of Animal Health

关键词: LH;    Pig;    TR-FIA;   

DOI  :  10.1292/jvms.69.1291

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(9)We validated a time-resolved fluoroimmunoassay (TR-FIA) using the lanthanide element europium as a non-radiological tracer for measuring luteinizing hormone (LH) in porcine peripheral blood. The dose-response curve of the reference standard ranged from 0.2048 to 50 ng/ml. Good parallelism was noted between the LH standard and plasma sample. The profile of LH throughout the estrous cycle, assessed by daily blood sampling, was consistent with the previous findings obtained by radioimmunoassays (RIAs). Moreover, the secretory patterns of pulsatile LH in the follicular phase and the preovulatory LH surge were also similar to those obtained in previous RIAs. We conclude that TR-FIA can be used to measure LH levels in porcine blood, with practical and convenient applications.

【 授权许可】

Unknown