Quasi-immune response of Penaeus japonicusto penaeid rod-shaped DNA virus (PRDV)

Diseases of Aquatic Organisms

K. Mushiake¹  L. Nonaka¹  C. A. Venegas¹  K. Muroga¹  T. Nishizawa¹ 

关键词: Penaeid acute viremia (PAV);    Penaeid rod-shaped DNA virus (PRDV);    White spot syndrome virus (WSSV);    Immune response;    Penaeus japonicus;   

DOI  :  10.3354/dao042083

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A quasi-immune response was demonstrated in kuruma prawn Penaeus japonicus infected naturally or experimentally with PRDV (penaeid rod-shaped DNA virus, also called white spot syndrome virus or WSSV), the causative agent of PAV(penaeid acute viremia). In the first step of this study, natural survivors 4 mo after a PAV outbreak demonstrated 94% relative percent survival (RPS) upon experimental PRDV challenge. Mortalities after challenge were confirmed by PRDV detection tobe due to PAV using a PCR method. In the second step, experimental PAV survivors were produced by intramuscular (IM) injection of PRDV into naive shrimp subsequently reared collectively in a tank (A group) or individually in chamber units (B group).Survival was 41 and 90% in the A and B groups, respectively. A subsequent IM re-challenge of these PRDV survivor groups with PRDV made 32 d after the first challenge revealed a protective response with high RPS of 77 and 64%, respectively. These highsurvival rates suggested that PAV survivors (natural or experimental) were able to resist PRDV infection and that the resistance was not due to selection of naturally resistant shrimp during a PAV outbreak, but due to enhancement of an immune-like system(quasi-immune response) after exposure to PRDV. No PRDV neutralizing activity was revealed in the serum of the 4 mo natural survivors of the PRDV outbreak. However, it was found in their serum 17 d after they had been experimentally challenged with PRDV.

【 授权许可】

Unknown