Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening

Diseases of Aquatic Organisms

Heping Dai¹  Lingfen Dai¹  Ruohong Zhao¹  Weizhou Chen¹  Chensong Jia¹  Xiaohua Zhang¹  Sean M. Hemmingsen¹  Nan Xiao¹  Li Yuan¹ 

关键词: White spot syndrome virus (WSSV);    Phage display;    Neutralizing antibody;    Single chain fragment variable regions (scFv) antibody;   

DOI  :  10.3354/dao072093

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease.

【 授权许可】

Unknown