In Vitro Cultivation of the Third and Fourth Stage Larvae of Angiostrongylus cantonensis

Journal of Veterinary Medical Science

Hidekazu HATA¹ 

关键词: Angiostrongylus cantonensis;    chemically defined medium;    in vitro culture;   

DOI  :  10.1292/jvms.55.345

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(3)The third and fourth stage larvae of Angiostrongylus cantonensis were cultured in various media. When the third stage larvae were cultured in RPMI 1640 supplemented with 20% fetal calf serum for four weeks, about 30% developed to the late third stage. On the other hand, when the fourth stage larvae recovered from rat brains were cultured in Waymouth's chemically defined medium (MB 752/1) for one week, the worms grew rapidly and 74% developed into young adults. The mean body length of the worms in Waymouth's medium showed a 1.4-fold increase in size compared with that before culture.

【 授权许可】

Unknown