Improvement of Preimplantation Development of InVitro-Fertilized Bovine Zygotes by Glucose Supplementation to a ChemicallyDefined Medium

Journal of Veterinary Medical Science

Hidenobu UMEKI⁷  Satoshi ADACHI⁷  Kyoko ICHIKAWA³  Hiroko UCHIYAMA¹  Etsushi KANEKO⁴  Shuji KOBAYASHI⁸  Nobutada SAKAGAMI⁴  Kiyoshi AKIYAMA⁴  Hiromichi TAMADA⁶  Kazuhisa TAKESHITA²  Osamu NISHINO⁵ 

[1] Miyazaki Prefectural Livestock Research Institute, Miyazaki 889�?4411, Japan;Yamaguchi Prefectural Livestock Experiment Station, Yamaguchi 759�?2221, Japan;Kochi Prefectural Livestock Research Institute, Kochi 789�?1233, Japan;Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243�?0417, Japan;Nara Prefectural Livestock Technology Center, Nara 633�?1302, Japan;Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 598�?8531, Japan;Ooita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878�?0201, Japan;National Livestock Breeding Center, Fukushima 961�?8511, Japan

关键词: bovine;    chemically defined medium;    embryo;    embryo culture;    glucose;   

DOI  :  10.1292/jvms.13-0543

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(1)The influences of glucosesupplementation on early development of bovine embryos in BSA-free synthetic oviduct fluidwere examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose eitherat 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in thegroup supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controlswithout glucose supplementation. The results suggest that appropriate amounts of glucosesupplemented to the medium at the specific stage of embryo culture may be useful for theproduction of bovine blastocysts.

【 授权许可】

Unknown