Enhancement of Exopolysaccharide Production of Lactobacillus fermentum TDS030603 by ModifyingCulture Conditions

Bioscience of Microbiota, Food and Health

Kenji FUKUDA¹  Kenji UCHIDA²  Tala SHI¹  Ni Putu Desy ARYANTINI¹  Tadasu URASHIMA¹ 

[1] Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan;Research Center, Yotsuba Milk Products Co., Ltd., Hokkaido 061-1264, Japan

关键词: chemically defined medium;    gene expression;    highly viscous exopolysaccharide;    probiotics;   

DOI  :  10.12938/bmfh.33.85

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

References(31)Cited-By(2)To optimize culture conditions that enhance production of a highly viscous exopolysaccharide of Lactobacillusfermentum TDS030603, a chemically defined medium was examined. The best yield was found to be 199 ± 23 mg/l when 48-hrcultivation was microaerobically performed at 30°C in the chemically defined medium supplemented with 5% glucose and 1% ammoniumcitrate without pH control. In response to the optimized exopolysaccharide production, the mRNA expression levels ofepsB, epsE, and epsG elevated significantly. Our results indicated that theoptimal C/N ratio and/or microaerobic condition can alter the expression levels of several exopolysaccharide biosynthesis-relatedgenes promoting the exopolysaccharide production yield.

【 授权许可】

Unknown