Protective Effects of Oral Microencapsulated Mycoplasma hyopneumoniae Vaccine Prepared by Co-Spray Drying Method

Journal of Veterinary Medical Science

K. S. YEH¹  M. J. PAN²  C. W. LIAO¹  C. N. WENG¹  J. H. LIN² 

[1] Division of Animal Medicine, Animal Technology Institute Taiwan;Department of Veterinary Medicine, National Taiwan University

关键词: co-spray drying;    microsphere;    Mycoplasma hyopneumoniae;    oral vaccine;   

DOI  :  10.1292/jvms.65.69

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(21)The efficacy of Mycoplasma hyopneumoniae oral vaccine was investigated in microsphere dosage form. A co-spray drying process was used to apply an encapsulating material, Eudragit L30 D-55, to microspheres containing Mycoplasma hyopneumoniae antigens. The microspheres were generally effective (>93%) with protein release at pH 7.4, but almost none were released at pH 1.2, for 3 hr in an in vitro dissolution test. An SPF-swine model was used to evaluate the effectiveness of the microspheres as an oral vaccine, and the related immune responses. The serum's systemic IgG against M. hyopneumoniae was evoked by ELISA analysis, after a 2nd immunization of all pigs. The vaccinated groups' mean lesion score was significantly lower after the Mycoplasma hyopneumoniae challenge than that of the nonvaccinated/challenged groups (P<0.05). This study strongly suggests that the oral microspheres vaccine prepared by a co-spray drying method can provide effective protection against M. hyopneumoniae infection in pigs.

【 授权许可】

Unknown