Oral immunization with recombinant protein antigen expressed in tobaccoagainst fish nervous necrosis virus

The Journal of Veterinary Medical Science

Tae Geum KIM¹  Ho Seong CHO²  Tae Jung KIM³  Ja Young SEO³  Sang Ik PARK³ 

[1] Center for Jeongup Industry-Academy-InstituteCooperation, Chonbuk National University, Jeonju 54896, Republic ofKorea;College of Veterinary Medicine and Bio-safety ResearchCenter, Chonbuk National University, Iksan 54596, Republic ofKorea;College of Veterinary Medicine, Chonnam NationalUniversity, Gwangju 61186, Republic of Korea

关键词: betanodavirus;    nervous necrosis virus;    oral vaccine;    tobacco;   

DOI  :  10.1292/jvms.16-0408

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Nervous necrosis virus (NNV), also known as betanodavirus, has been recently implicatedin mass mortalities of cultured marine fish. An effective vaccine is urgently needed toprotect fish against this virus. However, parenteral immunization methods are verystressful. Individual immunization for thousands of fish is very labor intensive andexpensive. Therefore, we expressed NNV coat protein in tobacco chloroplasts and used it asan oral vaccine to induce immunities in fish followed by challenges with NNV. Our resultsrevealed that mice (IgG and IgA) and fish (IgM) immunized with the oral vaccine developedsignificantly higher antibody titers against the NNV coat protein. Fish were partiallyprotected against viral challenge. Taken together, our results demonstrated that aplant-based vaccine could effectively induce immune response and protect groupers againstNNV. The present method could be used to develop oral fish vaccine in the future.

【 授权许可】

Unknown