Characterization of CD4-Positive Lymphocytes in the Antiviral Response of Olive Flounder (Paralichthys oliveceus) to Nervous Necrosis Virus

International Journal of Molecular Sciences

JaeWook Jung¹  SiWon Kim¹  JinHong Chun¹  AeRin Lee¹  TaeSung Jung¹  YoungRim Kim¹  Jaesung Kim¹  JungSeok Lee¹  JassyMary S. Lazarte¹  HyoungJun Kim²  KimD. Thompson³ 

[1] Laboratory of Aquatic Animal Diseases, Research Institute of Natural Science, College of Veterinary Medicine, Gyeongsang National University, 501 Jinju, Gyeongnam 52828, Korea;Laboratory of Aquatic Animal Quarantine, General Service Division, National Fishery Products Quality Management Service, Busan 49111, Korea;Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK;

关键词: CD4 T lymphocytes;    helper T cells;    monoclonal antibody;    nervous necrosis virus;    olive flounder;   

DOI  :  10.3390/ijms21114180

来源: DOAJ

【 摘 要 】

The presence of CD4 T lymphocytes has been described for several teleost species, while many of the main T cell subsets have not been characterized at a cellular level, because of a lack of suitable tools for their identification, e.g., monoclonal antibodies (mAbs) against cell markers. We previously described the tissue distribution and immune response related to CD3ε and CD4-1 T cells in olive flounder (Paralichthys oliveceus) in response to a viral infection. In the present study, we successfully produce an mAb against CD4-2 T lymphocytes from olive flounder and confirmed its specificity using immuno-blotting, immunofluorescence staining, flow cytometry analysis and reverse transcription polymerase chain reaction (RT-PCR). Using these mAbs, we were able to demonstrate that the CD3ε T cell populations contain both types of CD4⁺ cells, with the majority of the CD4 T cell subpopulations being CD4-1⁺/CD4-2⁺ cells, determined using two-color flow cytometry analysis. We also examined the functional activity of the CD4-1 and CD4-2 cells in vivo in response to a viral infection, with the numbers of both types of CD4 T cells increasing significantly during the virus infection. Collectively, these findings suggest that the CD4 T lymphocytes in olive flounder are equivalent to the helper T cells in mammals in terms of their properties and function, and it is the CD4-2 T lymphocytes rather than the CD4-1 T cells that play an important role in the Th1 immune response against viral infections in olive flounder.

【 授权许可】

Unknown