Production of Transgenic Bovine Cloned Embryos Using Piggybac Transposition

Journal of Veterinary Medical Science

Islam M. SAADELDIN¹  Su KIM¹  Song Jeon LEE³  Won Wu LEE³  Woo Jae CHOI¹  Byeong Chun LEE¹  Hyo Jun HAN²  Du Hee BANG²  Bong Han KIM¹  Goo JANG¹ 

[1] Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute, Seoul National University;Department of Chemistry, YonSei University;Embryo Research Center in Seoul Milk Coop.

关键词: bovine embryos;    GFP;    piggybac;    RFP;    SCNT;   

DOI  :  10.1292/jvms.11-0054

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(8)Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.

【 授权许可】

Unknown