Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis

FEBS Letters

Nakatsuji, Norio¹  Hasegawa, Kouichi¹ 

[1] Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan

关键词: Double gene transfection;    Enhancer blocking;    Insulator;    Fluorescent protein;    Transcriptional interference;    Transgenic mouse;    CAG;    cytomegalovirus enhancer and chicken β-actin promoter;    GFP;    green fluorescent protein;    RFP;    red fluorescent protein;    YFP;    yellow fluorescent protein;   

DOI  :  10.1016/S0014-5793(02)02761-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In transgenesis, the expression of two transgenes is often subject to mutual interference by each of the two expression cassettes when they are driven by different transcriptional regulatory elements in a single construct. To study this problem, we constructed vectors consisting of two expression units, one contains a strong ubiquitous promoter and the other contains a tissue-specific transcriptional element. The expression pattern of each transgene was examined in transfected cell lines and also in transgenic mice. In both cases, two expression units in a single construct were expressed in an independent manner and were controlled by their respective regulatory element only if we placed insulators at both ends of one expression unit. These results indicate that usage of insulators is a valuable tool for transfection of double gene constructs in transgenesis.

【 授权许可】

Unknown