The Detection of Bovine Lactoferrin Binding Protein on Trypanosoma brucei

Journal of Veterinary Medical Science

Ikuo IGARASHI²  Haruto KUMURA¹  Yukie ABE¹  Kei-ichi SHIMAZAKI¹  Tetsuya TANAKA¹  Noboru INOUE²  Woan-Sub KIM¹  Hideyuki NAGASAWA² 

[1] Dairy Science Laboratory, Graduate School of Agriculture, Hokkaido University;The Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

关键词: glyceraldehyde-3-phosphate dehydrogenase;    lactoferrin;    ovotransferrin;    transferrin;    Trypanosoma brucei;   

DOI  :  10.1292/jvms.66.619

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(29)Cited-By(13)Trypanosoma brucei, the causative agent of sleeping sickness in humans, requires transferrin (TF) for growth. Therefore, T. brucei has a TF receptor that allows it to obtain iron from TF. Lactoferrin (LF), a member of the TF family protein, is an iron-binding protein that is found in most biological fluids of mammals. LF has been shown to interact with some bacteria species by specific receptor-ligand binding. We examined the ability of T. brucei to bind bovine LF (bLF) by using a fluorescence test, streptavidin-biotin (SAB) microplate analysis, and far Western blotting using a biotin-streptavidin system. We found that bLF bound to components of T. brucei, and that bLF hydrolysate disrupted the sites responsible for binding to parasite proteins. Furthermore, bLF, human LF, bovine TF, and ovotransferrin bound same proteins of T. brucei, which exhibited molecular masses of 40 and 43 kDa. The N-terminal amino acid sequence of the 40 kDa bLF binding protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

【 授权许可】

Unknown