Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

Journal of Veterinary Medical Science

Takashi KUBO¹  Yohji OHNAMI²  Yoshiko OHIWA²  Koichi ORINO¹  Miho KUBOTA²  Motohiro KIKUCHI²  Naoki YAMADA²  Kiyotaka WATANABE¹  Shohei MIZOROKI¹ 

[1] Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University;Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

关键词: canine;    lactoferrin;    seminal plasma;    sperm;    transferrin;   

DOI  :  10.1292/jvms.65.679

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(4)Lactoferrin purified from canine seminal plasma by a three-step chromatography procedure had a molecular mass of 75.2 kDa and cross-reacted with antiserum to equine seminal plasma lactoferrin. Seminal plasma lactoferrin concentrations were determined by a competitive enzyme-linked immunosorbent assay (ELISA) by using rabbit anti-equine lactoferrin antibody and alkaline phosphatase-labeled goat anti-rabbit IgG antibody in 14 normal dogs and found to range from 12 to 197 μg/ml, with a mean value of 77 ± 59 μg/ml (the mean ± SD). Seminal plasma transferrin concentrations were determined by a sandwich ELISA with goat antibody to canine serum transferrin and alkaline phosphatase-conjugated goat anti-canine transferrin antibody and found to range from 0.32 to 12.6 μg/m l, with a mean value of 2.44 ± 3.25 μg/m l. The lactoferrin concentration significantly correlated with the sperm concentration (r=0.7025, P<0.01), but there was no significant correlation between the seminal plasma transferrin concentration and sperm density. These results indicate that seminal plasma lactoferrin, but not transferrin, reflects gonadal function.

【 授权许可】

Unknown