Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of São Paulo State, Brazil

Pesquisa Veterinária Brasileira

Langoni, Hélio²  Silva, Rodrigo Costa da²  Silva, Aristeu Vieira da¹  Tanaka, Erika Maemi²  Souza, Luiz Carlos de²  Lima, Vanessa Yuri de³ 

[1] Pontifícia Universidade Católica do Paraná, Umuarama, Brazil;Universidade Estadual Paulista, Botucatu, Brazil;Pontifícia Universidade Católica do Paraná, Toledo, Brazil

关键词: Toxoplasma gondii;    dogs;    antibodies;    risk factors;    infection;    public health.;   

DOI  :  10.1590/S0100-736X2010000200011

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Toxoplasmosis is caused by the obligate intracellular protozoan parasite Toxoplasma gondii and affects warm-blooded vertebrates, including pets and man. Dogs are epidemio-logically important since they act as sentinels for the infection in humans. The present study aimed to determine the presence of antibodies to T. gondii in 205 serum samples from dogs in Ubatuba, Sao Paulo state, Brazil, through indirect immunofluorescence reaction (IFAT), as well as the risk factors related to toxoplasmosis in the animals such as breed, age, sex, access to outdoors, homemade food ingestion, access to untreated water, and contact with rodents. Toxoplasmosis-positive samples accounted for 52/205 (25.4%), with titers ranging from 16 to 256. The serological results presented significant association (P<0.05) with homemade food ingestion (45/118; 38.1%; CI95% 29.9%-47.2%) (OR=7.0; CI95% 3.0-16.6), and with access to outdoors where those that do not have access to the street were prevalent (37/121; 30.6%; CI95% 23.1%-39.3%) (OR=0.5; CI95% 0.2-1.0). These results show that toxoplasmosis in this region is related to problems of sanitary education, mainly concerning the appropriate cooking of foods, since most positive animals did not show significant association with the presence of rodents or untreated water consumption but showed, instead association with ingestion of homemade food. Thus, toxoplasmosis is a public health problem in the studied region, and sanitary measures are needed to control the infection due to the strict relationship between man and dog and the presented risk factors. Index Terms: Toxoplasma gondii, dogs, antibodies, risk factors, infection, public health.RESUMOA toxoplasmose é causada por um protozoário parasita intracelular obrigatório, Toxoplasma gondii, e acomete vertebrados homeotérmicos incluindo animais de companhia e o homem. O cão apresenta importância epidemiológica por atuar como sentinela da infecção para o homem. O presente estudo objetivou determinar a ocorrência de anticorpos para T. gondii em 205 amostras de soro de cães do município de Ubatuba, SP, Brasil, pela reação de imunofluorescência indireta (RIFI), assim como os fatores de risco relacionados à infecção nos animais, como raça, idade, sexo, acesso a rua, ingestão de comida caseira, acesso a água não tratada e presença de roedores. 52/205 (25,4%) amostras foram positivas para toxoplasmose, com títulos variando de 16 a 256. Os resultados sorológicos apresentaram associação significativa (P<0,05) com consumo de comida caseira (45/118; 38,1%; IC95% 29,9%-47,2%) (OR=7,0; CI95% 3,0-16,6), e acesso a rua, em que aqueles que não tinham acesso a rua foram prevalentes (37/121; 30,6%; IC95% 23,1%-39,3%) (OR=0,5; CI95% 0,2-1,0). Estes resultados demonstram que a toxoplasmose na região está relacionada com problema de educação sanitária, principalmente quanto ao adequado cozimento dos alimentos, visto que a maioria dos animais positivos não apresentou associação significativa com presença de roedores ou consumo de água não tratada, porém os mesmos permaneciam em casa aos quais fora oferecida comida caseira. Portanto, a toxoplasmose consiste em um problema de saúde pública na região estudada, sendo necessárias medidas sanitárias para o controle da infecção, visto a estreita relação homem-cão e os fatores de risco presentes. Termos de Indexação: Toxoplasma gondii, cães, anticorpos, fatores de risco, infecção, saúde pública.  INTRODUCTION Toxoplasmosis is a zoonosis caused by the obligate intracellular parasite protozoan Toxoplasma gondii, which has worldwide distribution and infects most warm-blooded animals, including pets and man (Dubey 1994). The infection prevalence varies with the different species depending on occurrence area, socio-cultural habits, besides weather and geographic factors. Man and animals become infected by ingesting raw or poorly cooked meat containing tissue cysts (Dubey & Beattie 1988) and by ingesting oocysts present in contaminated feces of cats (definitive hosts) (Frenkel & Parker 1996, Lindsay et al. 1997, Clementino et al. 2007). Thus, dogs are epidemio-logically important since they act as sentinels of the infection to men, mainly errant animals or those with free access to other uncontrolled places preying on birds and rodents (Kawasoe 2000, Brito et al. 2002, Azevedo et al. 2005, Sedlak & Bartova 2006). Besides, dogs are good indicators of domestic environmental contamination, but in many parts of the world, including Brazil, dogs play a secondary role in T. gondii transmission because they are not food source for humans. These animals may be involved in the mechanical transmission of T. gondii to humans as reported by others (Frenkel & Parker 1996, Lindsay et al. 1997), mentioned above in the underlined sentence. The most important fact, however, is that dogs and man are exposed to the same sources of T. gondii (Dubey & Beattie 1988, Cabral et al. 1998, Garcia et al. 1999, Cañón-Franco et al. 2004, Jittapalapong et al. 2007). Thus, this study aimed to determine the frequency of antibodies to Toxoplasma gondii in dogs from Ubatuba, a coastal municipality in São Paulo State, Brazil, characte-rized by low hygienic-sanitary conditions associated with the tropical conditions of the Brazilian coast and the risk factors that favor the infection in animals.  MATERIALS AND METHODS Experimental design and sampling. This study was carried out in Ubatuba, a coastal municipality situated in the north of São Paulo State (23o26'S; 45o04'W), Southeastern region of Brazil, during a technical visit to the municipality, scheduled by the Municipal Secretary of Sanitary Vigilance, Ubatuba Municipal Council. Ubatuba is crossed by the Tropic of Capricorn (INMET 2005) (Fig.1) and presents rainfall index ranging approximately from 1600 to 3000 mm/year (PERH 2008); it is located at 250 km way from the State Capital and is surrounded by "Serra do Mar" and Atlantic Forest, in a 712 km2 total area and mean altitude of 835 m above the sea level, but presenting 800 to 1670 m elevations in its highest parts. The climate is humid tropical, with dominant winds from the south and southeast. Its estimated population is 75,008 inhabitants (IBGE 2008), with demographic density of 105.33 inhabitants/km2. The urbaniza-tion rate is 97.8%, with 22.8% of the population receiving sanitary treatment and 76.2% receiving treated water. This work was conducted as a transversal study, using blood sample collection to detect serum antibodies to Toxoplasma gondii and a questionnaire to survey epidemiological data. The presence of T. gondii antibodies was investigated in 205 dogs randomly selected among those from the studied municipality raised in houses and surroundings. Such number was determined based on the human population existent in the municipality and on the calculation of the proportion dog:man, 1:10 (Reichmann et al. 1999), resulting in a population of 7501 dogs. Considering a prevalence of 50% in the region, the software StatCalc, EpiInfo 3.1, at 95% confidence level and 7% error, was used to calculate the number of 191 animals, increased to 205 for safety reasons (Thrusfield 1995). There was no preference for sex, age or breed. All dog owners signed a consent and authorization term for the utilization of samples in this research. Questionnaire. Before blood sample collection, a compre-hensive questionnaire directed to the risk factors of toxoplas-mosis was given to the owners or those who raised or fed the dogs used in this study. The questionnaire included information about the dogs' breed, age, sex, homemade food ingestion, access to the street, and access to untreated water (both at home and on the streets), as well as the presence of rodents in the house or surroundings. Blood collection. Blood samples, 4 to 5mL, were collected through cephalic or jugular vein puncture. The collected blood was sent to Nupezo (Zoonosis Research Center) at the Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), Botucatu, SP, Brazil, where it was centrifuged at 1,600 x g for 10 minutes. Then, the serum was transferred to microtubes and stored at -20oC. Indirect immunofluorescence reaction (IFAT). Serum samples were investigated for the presence of antibodies to Toxoplasma gondii through indirect immunofluorescence reaction (IFAT), using the dilutions 1:16, 1:64, 1:256, 1:1024 and 1:4096, according to Camargo (1974). The used anti-dog IgG antibody, conjugated to fluorescein isothiocyanate, was kindly supplied by the Centro de Controle de Zoonoses in São Paulo (CCZ-SP) and diluted at 1:300 in Evans Blue solution 20mg%. Readings were done in a microscope "Zeiss SH250"; the sera reacting in the dilution 1:16 were considered positive (Silva et al. 1997). Statistical analysis. The contribution of the studied epidemiological variables to the result of the serological test to toxoplasmosis was verified through Chi-square or Fischer's Exact test using InStat 3 software, with a = 0.05.  RESULTS Indirect immunofluorescence results indicated 25.4% (52/205) positivity, with higher frequency in dogs from de coast of São Paulo State. The predominant titers were 16 in 8/52 samples (15.4%; CI95% 8.1-27.6%), 64 in 29/52 samples (55.8%; CI95% 42.3-68.4%), and 256 in 15/52 samples (28.8%; CI95% 18.3-42.4%). As shown in Table 1, there was no significant association between serological results and sex, age, access to untreated water or presence of rodents (P>0.05). On the other hand, serology was associated with non-access to the street (30.6%; CI95% 23.1-39.3%; OR=0.5; IC95% 0.2-1.0) and homemade food ingestion (38.1%; CI95% 29.9-47.2%; OR=7.0; IC95% 3.0-16.6).  DISCUSSION In the present study, 25.4% of the tested samples were positive by IFAT and were within the State range from 19.7 to 33.1% (Brito et al. 2002, Souza et al. 2003, Langoni et al. 2006) and the national range from 8.2 to 88.5% (Silva et al. 1997, Souza et al. 2003, Cañón-Franco et al. 2004, Azevedo et al. 2005, Romanelli et al. 2007, Figueredo et al. 2008, Da Silva et al. 2009, Santos et al. 2009). Such variation is mainly due to the large territorial extension of Brazil, with wide diversity of cultures, behaviors, socioeconomic and hygienic-sanitary conditions, besides the parasite intrinsic aspects like genetic variability. However, the disease worldwide prevalence in dogs is not too different from this range due to the same factors and is also frequently associated with religious conditions (Acha & Szyfres 2003). An example of this situation is observed in European, Asian and South-American continents. Sedlak & Bartova (2006) reported 107/413 (25.9%) prevalence in Czech Republic, and Wanha et al. (2005), 84/242 (35%) in Austria, whereas in Asian, Öncel et al. (2007) observed 77/150 (51.3%) in Tur

【 授权许可】

CC BY-NC