Immunofluorescence of the epizootic ulcerative syndrome pathogen, Aphanomyces invadans, using a monoclonal antibody

Diseases of Aquatic Organisms

James H. Lilley¹  Alexandra Adams¹  David J. C. Miles¹  Kim D. Thompson¹ 

关键词: Aphanomyces invadans;    Monoclonal antibody;    Immunohistochemistry;    IFAT;    Epizootic ulcerative syndrome;    Extracellular product;   

DOI  :  10.3354/dao055077

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A monoclonal antibody (MAb), designated 3gJC9, was raised against a protein antigen of Aphanomyces invadans, the oomycete pathogen that causes epizootic ulcerative syndrome (EUS). The antigen was expressed on the surface of hyphae andsecreted extracellularly. MAb 3gJC9 did not cross-react with other oomycete or fungal pathogens of fish, although it did react to the crayfish plague pathogen A. astaci. The MAb was used for immunofluorescent staining on histological sections offish infected with EUS, and was found to be more sensitive than conventional staining methods for detecting A. invadans. It thus has utility in confirming the case definition of EUS. It also revealed very small filamentous structures, thesignificance of which is unclear, but they may represent an early stage of infection, thus allowing earlier detection of the disease, since they are not detected using conventional staining methods.

【 授权许可】

Unknown