Expression Of Runx2 And Osteoblast Cell On The Periodontal Of Diabetes Mellitus Wistar Rat With Diet Extract Lemuru Fish Oils Treatment

12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry

生物科学;化学

Damaiyanti, W.D.^1 ; Parisihni, K.^1 ; Mulawarmanti, D.^1 ; Kurniawan, H.^2 ; Widyastuti^2

Oral Biology Department, Faculty of Dentistry, Universitas Hang Tuah, Jl Arif Rahman Hakim 150, Surabaya, Indonesia^1

Periodontic Department, Faculty of Dentistry, Universitas Hang Tuah, Jl Arif Rahman Hakim 150, Surabaya, Indonesia^2

关键词: Bone destruction;    Diabetes mellitus;    Fish oil;    Immunohistochemistry;    Innovative strategies;    Osteoblast cells;    Periodontal tissue;    RUNX2;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012057/pdf
DOI  :  10.1088/1755-1315/217/1/012057

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

The purpose of this study was to find an innovative strategy for controling progresivity of bone destruction on periodontal that caused by diabetic condition. Methods: Wistar rats sample are devided into 4 group; negative control group, 3 group with lemuru fish oil supplementation (4ml/kg of weight t, 8ml/kg of weight and 16ml/kg of weight). One week before treatment all group induced with STZ 65ml/KgBB and nicotinamide 110ml/KgBB to produced diabetes conditions. Immunohistochemistry slide of periodontal tissue was prepare after 3 weeks therapy. RUNX2 was count using HSCORE index, and osteoblast cell amount was count using image raster by optilab program. Results: Statistical analyses demonstrated a significant increase of RUNX2 expression in negative control group compare to treatment group (p