Susceptibility of the Australian freshwater crayfish Cherax destructor albidus to white spot syndrome virus (WSSV)

Diseases of Aquatic Organisms

Brett F. Edgerton¹ 

关键词: Freshwater crayfish;    Cherax destructor albidus;    White spot syndrome virus;    Susceptibility;    Import risk analysis;   

DOI  :  10.3354/dao059187

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Cherax destructor occurs naturally and/or is farmed in all Australian mainland states and territories and is of major cultural, economical and conservation significance. The aim of this study was to determine susceptibility of thecommercially important subspecies C. destructor albidus to white spot syndrome virus (WSSV), a hazard to crustaceans and currently considered to be exotic to Australia. In challenge tests by intramuscular injection, C. destructor albidusdisplayed a similar level of susceptibility to white spot disease (WSD) as Penaeus monodon (i.e. 100% mortality in 3 d). In one oral challenge test where C. destructor albidus was subjected to significant temperature stress, over 50% died ofsevere WSD within 14 d post challenge. All dead and moribund crayfish displayed histopathological lesions typical for WSD and gave positive results for WSSV in DNA dot blot hybridization tests. Survivors to 30 d (n = 3) showed no lesions and gavenegative dot blot test results. In a second oral challenge test without temperature stress, mortality was delayed but reached 75% by 30 d. However, no typical WSD lesions were observed in the dead, dying or surviving crayfish and dot blot test resultswere negative. The results suggested that C. destructor albidus would be less susceptible than P. monodon to WSSV exposure via natural routes of infection in farms and in the wild. This information may be useful for disease import riskanalysis for WSSV.

【 授权许可】

Unknown