Susceptibility of juvenile Macrobrachium rosenbergii to different doses of high and low virulence strains of white spot syndrome virus (WSSV)

Diseases of Aquatic Organisms

Patrick Sorgeloos¹  Mathieu Wille¹  Maurice B. Pensaert¹  João J. Dantas-Lima¹  Vo Van Tuan¹  Hans J. Nauwynck¹  Khuong Van Thuong¹  Mathias Corteel¹  Victoria Alday-Sanz¹ 

关键词: White spot syndrome virus;    WSSV;    Macrobrachium rosenbergii;    Resistance;    Susceptibility;   

DOI  :  10.3354/dao02496

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: As some literature on the susceptibility of different life stages of Macrobrachium rosenbergii to white spot syndrome virus (WSSV) is conflicting, the pathogenesis, infectivity and pathogenicity of 2 WSSV strains (Thai-1 and Viet) were investigated here in juveniles using conditions standardized for Penaeus vannamei. As with P. vannamei, juvenile M. rosenbergii (2 to 5 g) injected with a low dose of WSSV-Thai-1 or a high dose of WSSV-Viet developed comparable clinical pathology and numbers of infected cells within 1 to 2 d post-infection. In contrast, a low dose of WSSV-Viet capable of causing mortality in P. vannamei resulted in no detectable infection in M. rosenbergii. Mean prawn infectious dose 50% endpoints (PID₅₀ ml⁻¹) determined in M. rosenbergii were in the order of 100-fold higher for WSSV-Thai-1 (10^5.3±0.4 PID₅₀ ml⁻¹) than for WSSV-Viet (10^3.2±0.2 PID₅₀ ml⁻¹), with each of these being about 20-fold and 400-fold lower, respectively, than found previously in P. vannamei. The median lethal dose (LD₅₀ ml⁻¹) determined in M. rosenbergii was also far higher (~1000-fold) for WSSV-Thai-1 (10^5.4±0.4 LD₅₀ ml⁻¹) than for WSSV-Viet (10^2.3±0.3 LD₅₀ ml⁻¹). Based on these data, it is clear that juvenile M. rosenbergii are susceptible to WSSV infection, disease and mortality. In comparison to P. vannamei, however, juvenile M. rosenbergii appear more capable of resisting infection and disease, particularly in the case of a WSSV strain with lower apparent virulence.

【 授权许可】

Unknown