Comparison of infectious haematopoietic necrosis virus (IHNV) isolation on monolayers and in suspended cells

Diseases of Aquatic Organisms

V. Jen1  P. Hostnik¹ 

关键词: IHNV;    EPC;    Susceptibility;   

DOI  :  10.3354/dao040225

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A cell culture virus isolation procedure for infectious haematopoietic necrosis virus (IHNV) in the epithelioma papulosum cyprini cell line (EPC) is described. Ovarian fluid samples were collected from fish and tested for IHNV at 9 farms. Thesamples were inoculated in parallel on 24 h old EPC cell monolayers and in freshly trypsinized cells. The titre of the initial virus isolation and of first passages were compared using the 2 methods for each sample. Titres were consistently higher insuspended cells and this method also proved more sensitive for isolation of IHN virus from ovarian fluids of infected fish.

【 授权许可】

Unknown