Marek's Disease Virus Serotype 2 Glycoprotein I Gene : Nucleotide Sequence and Expression by a Recombinant Baculovirus

Journal of Veterinary Medical Science

Takeshi MIKAMI¹  Mitsuru ONO¹  Hyung-Kwan JANG¹  Jin-Shun CAI¹  Tae-Jong KIM¹  Yoshinori TSUSHIMA¹  Masahiro NIIKURA¹ 

关键词: cross-reactive epitope;    glycoprotein I;    Marek's disease virus serotype 2;    recombinant baculovirus;   

DOI  :  10.1292/jvms.58.11_1057

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(55)Cited-By(10)In the Marek's disease virus (MDV) serotype 2 (MDV2) genome, a gene equivalent to the glycoprotein I (gI) of other alphaherpesviruses was identified and sequenced. The primary translation product comprises 355 amino acids with a Mr of 38.4 kDa. The predicted amino acid sequence possesses several characteristics typical of membrane glycoproteins, including a N-terminal hydrophobic signal sequence, C-terminal transmembrane and cytoplasmic domains, and extra-cellular region containing three potential N-linked glycosylation sites. Compared to other MDV serotypes, MDV2 gI showed 49% identity with MDV1 gI, and 36% identity with HVT gI at the amino acid level. In transcriptional analyses, a 3.5 kb mRNA which starts between 56 and 147 bps upstream of the potential translational initiation codon of gI was identified as the gI-specific transcript. By a recombinant baculovirus, this potential gI encoding region was expressed as two specific products 45 and 43 kDa. Both products were susceptible to tunicamycin treatment, indicating that they were glycoprotein. Further, the expressed gI reacted with all chicken-antisera raised to each of the three serotypes of MDV (strains GA, SB-1, and FC126), suggesting that gI is expressed by all three serotypes of MDV in infected cells and conserves common antigenic epitope(s) beyond serotypes.

【 授权许可】

Unknown