Comparison of Two Different Nutritive Conditions against the Changes in Peripheral Blood Mononuclear Cells of Periparturient Dairy Cows

Journal of Veterinary Medical Science

Seiichi KAWAMURA³  Takaaki ANDO³  Masateru KOIWA¹  Hiromichi OHTSUKA³  Shigeru SATO⁵  Chika WATANABE³  Ryo ABE²  Masayuki KOHIRUIMAKI³  Daisaku WATANABE³  Tomohito HAYASHI²  Machiko MASUI⁴ 

[1] Department of Large Animal Clinical Sciences, Graduate School of Veterinary Medicine, Rakuno Gakuen University;Research Institute for Biological Sciences, Tokyo University of Science;School of Veterinary Medicine and Animal Sciences, Kitasato University;Kohiruimaki Animal Medical Service;Department of Miyagi Agricultural Mutual Relief Association

关键词: dairy cow;    immune function;    nutritive conditions;    periparturient;   

DOI  :  10.1292/jvms.68.1161

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(21)To clarify the relationship between cellular immune status and nutritive condition in periparturient dairy cows, feeding content, blood profiles, and immune condition were observed in cows from two dairy herds with different types of feed content. Immunological analyses such as leukocyte population and peripheral blood mononuclear cell (PBMC) mRNA of IFN-γ, TNF-α, IL-4, and IL-10, quantified by real-time RT-PCR were performed. With regard to feed content during dry periods, there were six cows in the herd with insufficient non-structural carbohydrate (NFC) intake (group I) and six cows in the herd with sufficient NFC intake (group II). Significantly lower levels of blood glucose were observed in group I between weeks -12 and 16 compared with group II. Serum cholesterol level was significantly lower in group I between weeks 2 and 10 than in group II. The numbers of CD3+ and CD4+ T cells in group I were significantly lower than those in group II in weeks 6 and 14. The numbers of CD21+ B cells were significantly lower in group I than in group II in weeks -16, -12, 2, and 10. On the other hand, the CD4+/CD8+ ratio in group II was significantly higher than group I between weeks 2 and 14. The IFNγ/IL-4 mRNA rate in group I was significantly lower than group II in week 6. We concluded that cellular immune depression occurrs after calving in dairy cows with low nutritional status in the periparturient period.

【 授权许可】

Unknown