Molecular Identification of a Recent Type of Canine Distemper Virus in Japan by Restriction Fragment Length Polymorphism

Journal of Veterinary Medical Science

Chieko KAI¹  Takeshi MIKAMI¹  Kenjiro OHASHI¹  Kazuya NAKAMURA¹  Kiyoko IWATSUKI¹ 

[1] Department of Veterinary Microbiology, Graduate School of Agricultural and Sciences, The University of Tokyo

关键词: canine distemper;    haemagglutinin;    RFLP;    RT-PCR;    vaccine;   

DOI  :  10.1292/jvms.60.1209

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(4)Restriction fragment length polymorphism analysis was used to differentiate recent field viruses of canine distemper virus (CDV) from vaccine strains. Virus genomes were amplified by using reverse transcriptase-polymerase chain reaction in part of the haemagglutinin gene. After digestion with EcoRV, the PCR products of recent field isolates were cut into two fragments that differ from the uncut form of old strains including all of vaccine strains. This method could be applied to fresh or stored brains, spleens and peripheral blood mononuclear cells of infected dogs. This molecular approach is useful for determining the causative agent of post-vaccinated CDV infection.

【 授权许可】

Unknown