Role of histone acetylation and DNA methylation in the maintenance of the imprinted expression of the H19 and Igf2 genes

FEBS Letters

Bruni, Carmelo B.³  Riccio, Andrea¹  Ungaro, Paola³  Vernucci, Maria³  Cerrato, Flavia³  Pikaart, Michael J.²  Pedone, Paolo V.¹ 

[1] Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli, via Arena 22, 81100 Caserta, Italy;Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA;Centro di Endocrinologia ed Oncologia Sperimentale, CNR, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, via S. Pansini 5, 80131 Naples, Italy

关键词: Histone acetylation;    DNA methylation;    Genomic imprinting;    Insulin-like growth factor-2;    H19;    Histone deacetylase inhibitors;    Igf2;    insulin-like growth factor-2;    5-azaC;    5-aza-2′-deoxycytidine;    TSA;    trichostatin;    UTR;    untranslated region;    PCR;    polymerase chain reaction;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    RFLP;    restriction fragment length polymorphism;   

DOI  :  10.1016/S0014-5793(99)01124-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

H19 and Igf2 are linked and reciprocally imprinted genes. We demonstrate that the histones associated with the paternally inherited and unexpressed H19 allele are less acetylated than those associated with the maternal expressed allele. Cell growth in the presence of inhibitors of either histone deacetylase or DNA methylation activated the silent Igf2 allele, whereas derepression of the silent HI9 allele required combined inhibition of DNA methylation and histone deacetylation. Our results indicate that histone acetylation as well as DNA methylation contribute to the somatic maintenance of H19 and Igf2 imprinting and that silencing of the imprinted alleles of these two genes is maintained via distinct mechanisms.

【 授权许可】

Unknown