Epigenetic modifications potentially controlling the allelic expression of imprinted genes in sunflower endosperm

BMC Plant Biology

Meiling Liu¹  Shuai Yu¹  Zhichao Zhang¹  Siqi Jiang¹  Yanye Ruan¹  Cong Li¹  Haoran Xia¹  Yue Zhou¹  Daqiu Sun¹  Yanshu Zhu¹  Xiaomei Dong²  Jing Li³  Yanbin Zhu⁴ 

[1] College of Bioscience and Biotechnology, Shenyang Agricultural University, 110866, Shenyang, Liaoning, China;Shenyang City Key Laboratory of Maize Genomic Selection Breeding, 110866, Shenyang, Liaoning, China;College of Bioscience and Biotechnology, Shenyang Agricultural University, 110866, Shenyang, Liaoning, China;Shenyang City Key Laboratory of Maize Genomic Selection Breeding, 110866, Shenyang, Liaoning, China;State Key Laboratory of Maize Bio-Breeding, Shenyang, China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China;State Key Laboratory of Maize Bio-Breeding, Shenyang, China;State Key Laboratory of the Northeast Crop Genetics and Breeding, Shenyang, China;

关键词: Genomic imprinting;    DNA methylation;    Endosperm;    Sunflower;   

DOI  :  10.1186/s12870-021-03344-4

来源: Springer

【 摘 要 】

BackgroundGenomic imprinting is an epigenetic phenomenon mainly occurs in endosperm of flowering plants. Genome-wide identification of imprinted genes have been completed in several dicot Cruciferous plant and monocot crops.ResultsHere, we analyzed global patterns of allelic gene expression in developing endosperm of sunflower which belongs to the composite family. Totally, 691 imprinted loci candidates were identified in 12 day-after-pollination sunflower endosperm including 79 maternally expressed genes (MEG) and 596 paternally expressed genes (PEG), 6 maternally expressed noncoding RNAs (MNC) and 10 paternally expressed noncoding RNAs (PNC). And a clear clustering of imprinted genes throughout the rapeseed genome was identified. Generally, imprinting in sunflower is conserved within a species, but intraspecific variation also was detected. Limited loci in sunflower are imprinted in other several different species. The DNA methylation pattern around imprinted genes were investigated in embryo and endosperm tissues. In CG context, the imprinted genes were significantly associated with differential methylated regions exhibiting hypomethylation in endosperm and hypermethylation in embryo, which indicated that the maternal demethylation in CG context potentially induce the genomic imprinting in endosperm.ConclusionOur study would be helpful for understanding of genomic imprinting in plants and provide potential basis for further research in imprinting in sunflower.

【 授权许可】

CC BY