The Effect of Oligosaccharides on the Production of Tumor Necrosis Factor-α by Macrophage-Like Cell Line J774/JA-4

Journal of Veterinary Medical Science

Eiichi MOMOTANI³  Fumio AMANO²  Kazuhiro YOSHIHARA¹  Satoko WATANABE³ 

[1] National Institute of Health;National Institute of Animal Health

关键词: macrophage;    oligosaccharide;    TNF;   

DOI  :  10.1292/jvms.60.519

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(3)Stimulation and modulation of tumor necrosis factor-α (TNF-α) production during treatment of the murine macrophage-like cell line J774/JA-4 with 25 oligosaccharides were studied. Direct stimulation of TNF-α production by oligosaccharides was measured with a cytotoxic assay using the L929 cell line. Twelve samples showed a significantly higher production (P ≤ 0.01) of TNF-α than the controls. Modulation of TNF-α production by treatment with oligosaccharides, followed by stimulation with lipopolysaccharide (LPS) from E. coli, was examined using the L929 bioassay system. In three samples TNF-α production increased significantly, but in four samples, production was reduced significantly (P ≤ 0.01). No samples showed modulation of growth or viability of L929 cells within the first 26 hr. The present results are useful in the application of these oligosaccharides which is potentially applicable in medical and food technology.

【 授权许可】

Unknown