Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes

Membranes

Takahiko Aoki¹  Kenji Chimura¹  Hikaru Sugiura¹  Yasuko Mizuno² 

[1] Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan; E-Mails:;Toray Research Centre, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan; E-Mail:

关键词: fish;    red blood cell membranes;    erythrocyte;    glycophorin;    oligosaccharide;    sialic acid;    N-glycolylneuraminic acid;   

DOI  :  10.3390/membranes4040764

来源: mdpi

【 摘 要 】

We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.