Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture*

Diseases of Aquatic Organisms

Jennifer L. Specker¹  Marta Gómez-Chiarri¹  Michael Mauel¹  David R. Nelson¹  Bruno Soffientino¹  Todd Gwaltney¹ 

关键词: Flounder infectious necrotizing enteritis (FINE);    Vibriosis;    Enteritis;    Paralichthys dentatus;    Vibrio carchariae;    Aquaculture;   

DOI  :  10.3354/dao038201

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: An epizootic causing mortality among cultured summer flounder Paralichthys dentatus occurred in summer of 1998 at a land-based facility on Narragansett Bay, Rhode Island, USA. The disease, flounder infectious necrotizing enteritis(FINE), was characterized by reddening around the anal area, distended abdomens filled with opaque serosanguineous fluid, enteritis and necrosis of the posterior intestine. In extreme cases of the disease, the posterior intestine was detached from theanus and was observed coming out the vent. The intestine of individuals that recovered from the disease ended in a blind-sac; the abdomens of these fish were distended, due to food and water inside the intestinal blind-sac. A bacterium was isolated fromascites fluid and kidney of moribund flounder and identified as the causative agent in challenge experiments. The pathogen was identified as Vibrio carchariae by morphological and biochemical characteristics and sequence of the 16S rRNA. TheLD₅₀ estimate was 5 x 10⁵ colony-forming units injected intraperitoneally into 100 to 200 g summer flounder.

【 授权许可】

Unknown