Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea

Virology Journal

Kyoung-Oh Cho²  Woo Song Lee³  Sang-Ik Park²  Su-Jin Park³  Young Bae Ryu³  Hyung-Jun Kwon³  Ju-Hwan Lee⁴  Kyu-Yeol Son²  Myra Hosmillo²  Deok-Song Kim²  Young-Ju Jeong¹  Ji-Yun Kim²  Eun-Hye Ryu²  Jun-Gyu Park²  Hyun-Jeong Kim²  Mia Madel Alfajaro² 

[1] Present address: Department of Clinical Pathology, College of Medicine, Seonam University, Namwon, 590-711, Republic of Korea;Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Republic of Korea;Eco- Friendly Biomaterial Research Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongup, 580- 185, Republic of Korea;Chonnam National University Veterinary Teaching Hospital, Gwangju, 500-757, Republic of Korea

关键词: Anti-rotaviral drug;    Glycyrrhiza uralensis extract;    Enteritis;    Rotavirus;   

Others  :  1152725
DOI  :  10.1186/1743-422X-9-310

 received in 2012-07-03, accepted in 2012-12-10,  发布年份 2012

【 摘 要 】

Background

Since rotavirus is one of the leading pathogens that cause severe gastroenteritis and represents a serious threat to human and animal health, researchers have been searching for cheap, safe, and effective anti-rotaviral drugs. There is a widespread of interest in using natural products as antiviral agents, and among them, licorice derived from Glycyrrhiza spp. has exerted antiviral properties against several viruses. In this study, anti-rotaviral efficacy of Glycyrrhiza uralensis extract (GUE) as an effective and cheaper remedy without side-effects was evaluated in colostrums-deprived piglets after induction of rotavirus diarrhea.

Methods

Colostrums-deprived piglets were inoculated with porcine rotavirus K85 (G5P[7]) strain. On the onset of diarrhea, piglets were treated with different concentration of GUE. To evaluate the antiviral efficacy of GUE, fecal consistency score, fecal virus shedding and histological changes of the small intestine, mRNA expression levels of inflammation-related cytokines (IL8, IL10, IFN-β, IFN-γ and TNF-α), signaling molecules (p38 and JNK), and transcription factor (NFκB) in the small intestine and spleen were determined.

Results

Among the dosages (100-400 mg/ml) administrated to animals, 400 mg/ml of GUE cured diarrhea, and markedly improved small intestinal lesion score and fecal virus shedding. mRNA expression levels of inflammation-related cytokines (IL8, IL10, IFN-β, IFN-γ and TNF-α), signaling molecules (p38 and JNK), and transcription factor (NFκB) in the small intestine and spleen were markedly increased in animals with RVA-induced diarrhea, but dose- dependently decreased in GUE treated animals after RVA-induced diarrhea.

Conclusions

GUE cures rotaviral enteritis by coordinating antiviral and anti-inflammatory effects. Therapy of this herbal medicine can be a viable medication for curing rotaviral enteritis in animals and humans.

【 授权许可】

   
2012 Alfajaro et al.; licensee BioMed Central Ltd.

【 图 表 】

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