trans-RESVERATROL AND ANTIOXIDANT ACTIVITY OF GRAPE (Vitis vinifera sp) BYPRODUCTS

Brazilian Journal of Food Research

Marcela Boroski¹  Milena Keller Bulla³  Carmen Maria Donaduzzi²  Marcelo Shigueru Kamei²  Márcia Lina Mitsui³  Diógenes Aparício Garcia Cortez³  Lucia Elaine Ranieri Cortez³ 

[1] Universidade Federal da Integraçãoo Latino Americana;Prati-Donaduzzi & Cia Ltda.Toledo, Paraná, Brazil.;Universidade Estadual de Maringá (UEM), Program of Post-Graduation in Pharmaceutical Sciences. Maringá, Paraná, Brazil.

关键词: Grape;    polyphenols;    residue;    seeds;    husks.;   

DOI  :  

学科分类：解剖和生理学

来源: Universidade Tecnica Federal do Parana

【 摘 要 】

Polyphenols from agroindustries byproducts have great potential as a source of antioxidant compounds. The present study evaluated the antioxidant activity and polyphenol content of the husks and seeds of grapes obtained from a winery of the produce wine. The hydroalcoholic extract (HAE) of the husks was obtained through an exhaustive maceration process. Seeds were extracted in a Soxhlet system, mixed with hydroalcoholic solution and the dried extract was partitioned into solvents to yield the chloroform fraction (CLF), ethyl acetate fraction (EAF), and butanol fraction (BUF). The EAF and HAE had phenolic equivalents of 200.76 and 320.64 mg of gallic acid per gram of extract, respectively. The HAE showed higher antioxidant activity than the fractions in the phosphomolybdenum method, the same in DPPH assay, with IC 50of 16.82 mg/mL, andβ -carotene/linoleate system, with antioxidant activity of 82.09%. High performance liquid chromatography (HPLC) method was developed and validated for quantifyingtrans -resveratrol in the HAE. The method showed linearity (2.5-7.5 µg/mL), correlation coefficient of 0.9907, precision and accuracy (resveratrol recovery = 101.02%). The HAE had atrans -resveratrol concentration of 17.33 µg/mg. The results of the validation of the HPLC method were satisfactory, which allowed analysis of the extract with precision and accuracy. 

【 授权许可】

Unknown