Prevalence of canine coronavirus (CCoV) in dog in Japan: detection of CCoV RNA and retrospective serological analysis

Journal of Veterinary Medical Science

Kumi SATOH¹  Tsutomu HOHDATSU¹  Tomomi TAKANO¹  Saya YAMASHITA¹  Tomoyoshi DOKI¹  Michiko MURATA-OHKUBO¹ 

[1] Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034�?8628, Japan

关键词: canine coronavirus;    RT-PCR;    serological prevalence;    serotype;   

DOI  :  10.1292/jvms.15-0347

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)We collected rectal swabs from dogs in Japan during 2011 to 2014, and canine coronavirus (CCoV) nucleocapsid gene was detected by RT-PCR. The relationship between CCoV infection and the manifestation of diarrhea symptoms was investigated, and a correlation was noted (df=1, χ2=8.90, P<0.005). The types of CCoV detected in samples from CCoV-infected dogs were CCoV-I in 88.9% and CCoV-II in 7.4%, respectively. We retrospectively investigated the seroprevalence of CCoV-I in dogs in Japan during 1998 to 2006. The sera were tested with a neutralizing antibody test. In the absence of CCoV-I laboratory strain, we used feline coronavirus (FCoV)-I that shares high sequence homology in the S protein with CCoV-I. 77.7% of the sera were positive for neutralizing anti-FCoV-I antibodies.

【 授权许可】

Unknown